Friday, December 20, 2013

Cell Communication Lab

Purpose 
Our purpose in this lab was to see how cells communicate among each other to reproduce and send signals without moving
Introduction
Cells communicate when and where to breed through quorum sensing. This is how yeast reproduces. In the lab we were seeing how each cell responds to this. 

Methods
We took cultured yeast and labeled at as a, alpha, and mixed, added broth and santatized water, we took it under the microscope and looked at the particles of yeast through the microscope after incriminats of 30 minutes, 24 hours and 48 hours and counted to see how much they increased. 
Data
In the lab, we noticed a pattern of yeast growth in the alpha-type in both single and budding haploid. But in the a-type, there is no pattern of growth. 


Graphs and Charts

Discussion
In this lab our data varied between the a type, alpha and the mixed. The most particles were present in the mixed, as expected. The third reading of ours type culture was screwed up because what was seen on the slide was spread out, moving the spread of cells in the third view. They weren't even throughout the slide or the test tube we were keeping them in. If we were to increase validity in our experiment we would have more than one sample of each culture to see variations in each. In our graph each "reading" (1, 2,3, and 4) they each represented different amount of time reading one was as soon as we put everything together in the tube, reading 2 was after a half an hour, 3 was after 24 hours, and 4 was 48 hours. We would extend the experiment over a longer amount of time and calculate the amount of percentage increase after each day to see how much they increase per day over a week of time
Conclusion
The cells will continue to divide over time. This leads to most haploid cells that then come together to form the budding. The yeast will continue to divide and grow indefinitely. 
References 
The lab
The book


2 comments:

  1. References should be a little more detailed. Your discussion should have addressed the laboratory inquiry questions as well and included information on G coupled receptors and how they work. Nice data table and graphs.

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  2. The introduction should have included a discussion on G coupled protein receptors and the lab was not studying quorum sensing but rather signal transduction pathways and how cells respond to external stimuli. Your conclusion did not state whether or not your hypothesis was supported or refuted and did not include any potential sources of errors. The discussion was to also include answers to the lab analysis questions included in the lab protocol.

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