Our purpose in this lab was to see how cells communicate among each other to reproduce and send signals without moving
Cells communicate when and where to breed through quorum sensing. This is how yeast reproduces. In the lab we were seeing how each cell responds to this.
We took cultured yeast and labeled at as a, alpha, and mixed, added broth and santatized water, we took it under the microscope and looked at the particles of yeast through the microscope after incriminats of 30 minutes, 24 hours and 48 hours and counted to see how much they increased.
In the lab, we noticed a pattern of yeast growth in the alpha-type in both single and budding haploid. But in the a-type, there is no pattern of growth.
Graphs and Charts
In this lab our data varied between the a type, alpha and the mixed. The most particles were present in the mixed, as expected. The third reading of ours type culture was screwed up because what was seen on the slide was spread out, moving the spread of cells in the third view. They weren't even throughout the slide or the test tube we were keeping them in. If we were to increase validity in our experiment we would have more than one sample of each culture to see variations in each. In our graph each "reading" (1, 2,3, and 4) they each represented different amount of time reading one was as soon as we put everything together in the tube, reading 2 was after a half an hour, 3 was after 24 hours, and 4 was 48 hours. We would extend the experiment over a longer amount of time and calculate the amount of percentage increase after each day to see how much they increase per day over a week of time
The cells will continue to divide over time. This leads to most haploid cells that then come together to form the budding. The yeast will continue to divide and grow indefinitely.