Monday, November 18, 2013

Cell Respiration Lab

The purpose was to compare the co2 emissions of germinating to dormant seeds. Also it was to determine the effect that temperature has on the cellular respiration. 

In this lab we measured the co2 that the germinating corn seeds produced. They are producing co2 because they are undergoing cellular respiration. A dormant seed has all the materials and food it needs to become a plant. When the seed becomes moist, the enzymes inside it start facilitating cellular respiration to make all the energy that the plant will need until it can go through photosynthesis.

In this lab we took the already germinated seeds and measured the amount of CO2 with the monitor to see what had the highest rate if respiration in ten minutes. Our group did corn, and we put it in ice cold water, to see what effect tempurature had on the rate of respiration. we used glass beads as a control group to make surety monitor was working properly.

In our experiment the room was 24 degrees Celsius, and the cold water was 20 degrees celcius. The germinated seeds respirated at .66274 (ppm/s), and the germinated seeds in cold water respirated more at .68034 (ppm/s). The non germinated seeds respirated a ton less at .25721 (ppm/s). 

Graphs and Charts

For the glass beads and the the non germinating seeds, both remained constant, neither showing any increase in CO2 output, which was to be expected. The germinating seeds at 24 degrees Celsius had a rate of .66 ppm/s while the cooler seeds at 20 degrees Celsius hade a rate of .68 ppm/s. This might not be accurate because the seeds at 20 degrees were not recorded for the full 10 mins. After 320 seconds, our group ran out of time. The rate of CO2 the seed produced could have slowed and ended with a slower rate after 10 minutes. If doing this experiment again, we would run each trial to the full time length. We would also soak the seeds in the ice water longer, further reducing their temperature. For the most part, the data did represent what we thought would be the results of the experiment. The glass beads are not alive, therefor not producing CO2. The no germinating seeds are not growing or using energy, so the would have no need to go through the process of cell respiration. Both germinating seeds would be going through cell respiration because they are growing. The cold seeds may have been producing more CO2 because they are trying to stay alive in the cold environment, and go through more cellular respiration to produce more heat. If I the data is inaccurate and the respiration rate should have been decreasing, it could be because the cold slowed the metabolic process down, affecting the CO2 output.

The answers to the questions that we were finding were that the germinating seeds gave off more co2  than the dormant seeds, which means that they were respirating more. Also, the seeds germinated more at lower temperatures. 


1 comment:

  1. Nice Job. Please place captions/labels under your methods pictures. This will make it easier for the reader to follow.